Objective This paper aims at establishing an inductively coupled plasma mass specrometry(ICP-MS)method for quantification and evaluation of iodine in human urine and serum in routine clinical laboratory.Methods This study was methodology validation research on iodine evaluation using ICP-MS.Ammonia,isopropanol and ultrapure water were mixed at certain ratio to Objective This paper aims at establishing an inductively coupled plasma mass specrometry (ICP-MS) method for quantification and evaluation of iodine in human urine and serum in routine clinical laboratory. Methods This study was methodology validation research on iodine evaluation using ICP-MS. Ammonia , isopropanol and ultrapure water were mixed at certain ratio to