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目的观察糖尿病不同时期视网膜突触后致密物蛋白质95(PSD95)的蛋白表达水平以及视网膜突触超微结构的变化。方法雄性3月龄SD大鼠56只,随机分为模型组和对照组,分别腹腔内注射链脲佐菌素(STZ)和0.1mol/L枸橼酸-枸橼酸钠缓冲液,于4、8、12周取眼球,入4%多聚甲醛后固定2h,冷冻切片厚20μm,采用免疫组织化学检测PSD95蛋白表达的变化,显微镜观察视网膜全层并摄片,图像分析;透射电镜观察视网膜突触并摄片。结果 PSD95蛋白表达在糖尿病第8周时开始升高,至12周时升高具有显著意义;视网膜内丛状层的突触结构中的突触间隙增大,突触后致密物厚度变薄,突触活性区长度变长。结论 PSD95的蛋白表达水平以及视网膜突触超微结构的改变可能与糖尿病视网膜病变的发展有关。
Objective To observe the protein expression of retinal postsynaptic density protein 95 (PSD95) and the ultrastructure of retina in different periods of diabetes mellitus. Methods Fifty-six male SD rats of 3 months old were randomly divided into model group and control group. STZ and 0.1 mol / L citrate-citrate buffer solution were intraperitoneally injected respectively. , Eyeballs were taken at 8 and 12 weeks, fixed for 2 hours after adding 4% paraformaldehyde, frozen section thickness was 20μm, the expression of PSD95 protein was detected by immunohistochemistry, the whole retina was observed by microscope and image analysis was performed. The retina was observed by transmission electron microscope Synapse and radiography. Results The expression of PSD95 protein began to increase at the 8th week of diabetes mellitus, and increased significantly at 12 weeks. The synaptic cleft in the synaptic structure of the intra-retinal plexiform layer increased and the thickness of the postsynaptic density became thinner. The length of the active region becomes longer. Conclusion The expression of PSD95 protein and the ultrastructure of retina synapse may be related to the development of diabetic retinopathy.