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以3个优良甘蓝品种为试材,进行了游离小孢子培养和胚状体植株再生技术研究,以期探讨不同甘蓝材料的基因型、胚状体类型、不定芽大小、烯效唑对不定芽诱导和生根的影响。结果表明:甘蓝子叶型胚容易发育单芽,鱼雷型胚和球型胚容易形成丛生芽;且单芽率和丛生芽率在不同基因型之间存在差异。高度大于2cm的不定芽转接到生根培养基上后生长速度先慢后快,生根数少且细长;小于2cm的不定芽生长速度先快后慢,生根数多且粗壮。小孢子不定芽在添加0.50mg/L烯效唑的MS+0.3mg/L NAA生根培养基中生根率最高且根系生长健壮,移栽成活率高达95.6%。
Three elite cabbage cultivars were used as materials to study the microspore culture and plantlet regeneration of embryoids. The purpose of this study was to investigate the genotype, embryoid body type, adventitious bud size, And rooting effect. The results showed that the single shoots were easy to develop in the cotyledon of Brassica oleracea, and the torpedo and globular embryos were easy to form the clustered buds. The single bud rate and clustered bud rate differed among different genotypes. The adventitious buds with a height of more than 2 cm were transferred to the rooting medium, the growth speed of the adventitious buds was slowed down first and then quickly, and the rooting number was small and slender. The adventitious buds less than 2 cm grew quickly and then slowly, with more rooting and thick stubble. The microspore adventitious buds had the highest rooting rate and robust root growth in MS + 0.3mg / L NAA medium supplemented with 0.50mg / L uniconazole, and the survival rate of transplanting was as high as 95.6%.