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本实验采用固体药物埋藏的方法,将阿托品、六烃季铵分别埋藏于大鼠下丘脑室旁核(PVH)附近,将阿托品分别埋藏于腹侧海马、背侧海马和丘脑。以麻醉状态下大鼠坐骨神经损伤性刺激(神经源性应激)作为应激条件,血浆皮质酮浓度变化作为观察指标。结果为:阿托品和六烃季铵分别埋藏于PVH后5d,坐骨神经损伤性刺激后30和60min血浆皮质酮浓度的升高,均受到显著的抑制,皮质酮总反应量也较埋药前明显下降(P<0.01)。将阿托品埋藏于腹侧海马后5 d,刺激后血浆皮质酮反应,也受到一定程度的抑制。而阿托品埋藏于背侧海马和丘脑,对损伤性应激引起的血浆皮质酮变化,未见显著影响(P>0.05)。结果表明,脑内PVH和腹侧海马内有胆碱能接受系统,对损伤性应激反应具有调节作用,而且这一作用存在着部位特异性。
In this experiment, atropine and hexachloroquaternium were buried in the vicinity of the hypothalamic paraventricular nucleus (PVH) in rats. The atropine was buried in ventral hippocampus, dorsal hippocampus and thalamus respectively. The anesthetic state of sciatic nerve injury (neurogenic stress) rats as stress conditions, plasma corticosterone concentration as an indicator. The results were as follows: 5 days after embedding of atropine and hexachloroquaternium in PVH, the plasma corticosterone concentrations were significantly inhibited at 30 and 60 minutes after sciatic nerve injury, and the total corticosterone reaction was also significantly lower than that before embedding (P <0.01). The atropine buried in the ventral hippocampus 5 d after stimulation of plasma corticosterone response, but also to some extent inhibition. The atropine buried in the dorsal hippocampus and thalamus, on the damage caused by corticosterone stress changes, no significant effect (P> 0.05). The results show that there are cholinergic systems in the PVH and ventral hippocampus of the brain, which have a regulatory effect on the damage-induced stress response. Moreover, this effect is site-specific.