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目的研究系统性红斑狼疮(SLE)患者外周血Vα24-Vβll自然杀伤T细胞(NKT)细胞数量变化和功能状态在致病中的作用。方法采用流式细胞仪检测32例SLE患者和30名正常对照组Vα24-Vβ11NKT细胞数量以及体外活化前后表达血细胞分化抗原(CD)69和白细胞介素-4(IL—4)、Ⅱ型干扰素(IFN-γ)的水平。结果SLE患者外周血Vα24-Vβ11NKT细胞数量(0.4%±0.25%)低于正常对照组(1.07%±0.23%,P<0.01),NKT细胞体外活化前CD69表达为5.26%±2.12%,正常对照组为11.47%±2.86%(P<0.05);活化后表达CD69为56.61%±0.47%,正常对照组为96.71%±0.33%(P<0.01)。SLE患者NKT细胞活化前其胞浆内IL-4含量为32.46±5.49pg/ml,正常对照组为12.21±3.31pg/ml,活化后其胞浆内IL-4含量为48.38±8.53pg/ml,正常对照为26.93±6.84pg/ml(均P<0.05),而IFN-γ,含量活化后为19.32±6.45pg/ml,正常对照组为33.65±11.91pg/m1(P<0.05)。结论SLE发病可能与NKT细胞数量的低下及功能严重失调相关。
Objective To study the changes of Vα24-Vβll natural killer T (NKT) cells in peripheral blood of patients with systemic lupus erythematosus (SLE) and the role of functional status in pathogenicity. Methods The number of Vα24-Vβ11NKT cells in 32 patients with SLE and 30 normal controls were measured by flow cytometry. The expressions of CD 69 and IL-4, type II interferon (IFN-γ) levels. Results The number of Vα24-Vβ11NKT cells in peripheral blood of SLE patients (0.4% ± 0.25%) was lower than that of the normal controls (1.07% ± 0.23%, P <0.01). The expression of CD69 in NKT cells was 5.26% ± 2.12% Group (11.47% ± 2.86%, P <0.05). The expression of CD69 was 56.61% ± 0.47% in the control group and 96.71% ± 0.33% in the normal control group (P <0.01). The level of IL-4 in cytoplasm of SLE patients before activation was 32.46 ± 5.49pg / ml, the normal control group was 12.21 ± 3.31pg / ml, and the cytoplasm IL-4 content was 48.38 ± 8.53pg / ml , The normal control group was 26.93 ± 6.84pg / ml (all P <0.05), while the level of IFN-γ was 19.32 ± 6.45pg / ml in the control group and 33.65 ± 11.91pg / ml in the normal control group (P <0.05). Conclusion The pathogenesis of SLE may be related to the low number of NKT cells and severe dysfunction.