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本文作者为了提高试验的可复验性,进行了此项研究。缓冲液:磷酸缓冲盐水(PBS)的成分含有0.06M的磷酸氢二钠和磷酸二氢钠及0.74%的氯化钠,加入叠氮化钠(0.1%)为防腐剂,缓冲液的pH为6.9。红细胞的制备:将收集的1日龄或2日龄的人体“O”细胞,放于改良的Alsever氏溶液(葡萄糖20.5克,枸橼酸钠8克,氯化钠4.2克,枸橼酸0.55克,蒸馏水1,000毫升,pH6.1)用PBS洗3次,制成4%的PBS细
The authors conducted this study to improve the test retest. Buffer: The phosphate buffered saline (PBS) consisted of 0.06M sodium phosphate dibasic and sodium dihydrogen phosphate and 0.74% sodium chloride. Sodium azide (0.1%) was added as a preservative and the pH of the buffer was 6.9. Preparation of Erythrocytes: The collected 1-day-old or 2-day-old human “O” cells were placed in a modified Alsever’s solution (20.5 g of glucose, 8 g of sodium citrate, 4.2 g of sodium chloride, 0.55 of citric acid 1,000 ml of distilled water, pH 6.1) was washed three times with PBS to make 4% PBS fine