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以云纹石斑鱼(Epinephelus moara)♀为母本,七带石斑鱼(E.septemfasciatus)♂为父本进行种间远缘杂交,系统观察了杂交子一代胚胎及仔稚幼鱼的生长发育及形态特征。对杂交子一代的胚胎发育按卵裂期、囊胚期、原肠胚期、神经胚期和器官形成期各阶段进行观察,在水温21～22.5℃、盐度29、pH 8～8.1的海水中,整个胚胎发育过程经35 h 20min完成。根据在卵黄囊期、第二背鳍棘和腹鳍棘生长消退过程、鳞片生长、体表色素变化的不同,观察比较了胚后发育的仔鱼期、稚鱼期、幼鱼期形态特征。鱼苗培育至58 d后完成了稚鱼至幼鱼的变态:初孵仔鱼4 d以前为内源性营养(卵黄囊仔鱼);4 d后开口摄食,为外源性营养;(42±2)d基本完成变态进入稚鱼阶段,(58±4)d全身被鳞,进入幼鱼期。同时,用同一母本(云纹石斑鱼)同批卵与同种雄鱼采集的精子授精后获得的受精卵作为对照组,进行孵化和仔稚幼鱼培育,与杂交子一代进行比较,结果发现杂交子一代在仔稚幼鱼阶段生长速度快于云纹石斑鱼,与七带石斑鱼相近。
Epinephelus moara ♀ as the female parent, seven fish grouper (E.septemfasciatus) ♂ as the father of interspecific distant hybridization, the system observed hybrid embryo and embryonic juvenile juvenile growth Development and morphological characteristics. Embryonic development of the hybrids was observed at each stage of cleavage stage, blastocyst stage, gastrulation stage, neuroblastoma stage and organ formation stage. Seawater water of 21-22.5 ° C, salinity 29, pH 8-8.1 In the whole embryo development process completed by 35 h 20min. According to the process of regrowth, the growth of scales and the change of body pigment in the yolk sac, the second dorsal fin spine and pelvic spines, morphological characteristics of larval stage, juvenile stage and juvenile stage were observed and compared. After the fish fry were incubated for 58 days, the metamorphosis of juvenile to juvenile fish was completed. The newly hatched larvae were endogenous nutrition (yolk sac larvae) 4 d before, and were exuded after 4 days. (42 ± 2) d basically metamorphosis into the juvenile stage, (58 ± 4) d body was scaly, into the juvenile stage. At the same time, the fertilized eggs collected from the sperm of the same female (Moire grouper) and the same males were used as the control group, hatched and juvenile larvae were incubated, compared with the hybrid generation, The results showed that the hybrids grew more rapidly in juvenile juveniles than moorhen groupers, similar to the seven-band grouper.