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利用肿瘤细胞表面抗原G250的单克隆抗体G250mAb和聚乙二醇(PEG)修饰非病毒基因载体聚乙烯亚胺(PEI),获得肿瘤靶向基因载体G250mAb-PEI-PEG.该载体对HeLa细胞的基因转染效率达到70%,远远高于对非肿瘤细胞NIH3T330%的转染效率.通过该基因载体将Nucleostemin(NS)基因的特异性短发夹RNA(short hairpin RNA,shRNA)高效率地转入靶细胞HeLa,RNA干扰下调了细胞中NS的表达,显著抑制了HeLa细胞的增殖能力,引起HeLa细胞凋亡,而对NIH3T3细胞的增殖和活性没有显著影响.研究结果表明,非病毒基因载体G250mAb-PEI-PEG可靶向性、高效率的转染HeLa细胞,应用于基因治疗,这对于宫颈癌的临床治疗具有重要的意义.
The tumor-targeting gene vector G250mAb-PEI-PEG was obtained by modifying the non-viral gene carrier polyethyleneimine (PEI) with monoclonal antibody G250mAb and polyethylene glycol (PEG) of tumor cell surface antigen G250. The gene transfection efficiency is up to 70%, which is much higher than the transfection efficiency to non-tumor cell NIH3T330% .The specific short hairpin RNA (shRNA) of Nucleostemin (NS) gene is highly efficient Transfected into HeLa target cells, RNA interference downregulated the expression of NS in the cells, significantly inhibited the proliferation of HeLa cells and caused the apoptosis of HeLa cells, but had no significant effect on the proliferation and activity of NIH3T3 cells.The results showed that non-viral genes Carrier G250mAb-PEI-PEG can be targeted and efficient transfected HeLa cells for gene therapy, which is of great significance for the clinical treatment of cervical cancer.