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目的探讨KAI1基因对MHCC97-H肝癌细胞粘附力的影响,从而推测KAI1基因影响MHCC97-H肝癌细胞侵袭转移的机制。方法采用微管吸吮技术研究我们前已转染人类KAI1全长正、反义结构基因的人肝癌MHCC97-H细胞粘附力。结果在纤维连接蛋白(fibronectin,FN)裱衬表面,利用微管吸吮系统测得的各组肝癌细胞的粘附力分别为MHCC97-H-S组(678·4±101·8),MHCC97-H-AS组(1083·6±113·8),MHCC97-H-pCI组(853·0±105·4)及MHCC97-H亲本细胞组(834·6±130·5)(单位为10-10N,细胞数为25)。与MHCC97-H亲本细胞组比较,MHCC97-H-S组细胞对FN的粘附力显著下降(P<0·01),MHCC97-H-AS组细胞对FN的粘附力显著增加(P<0·01),而MHCC97-H-pCI组细胞对FN的粘附力无明显变化(P>0·05)。结论KAI1基因可能降低肝癌细胞对细胞外基质FN的粘附力,从而抑制转移的初始步骤,进而抑制MHCC97-H肝癌细胞的侵袭和转移。
Objective To investigate the effect of KAI1 gene on the adhesion of MHCC97-H hepatocellular carcinoma cells, and to infer the mechanism by which KAI1 affects the invasion and metastasis of MHCC97-H hepatocellular carcinoma cells. Methods The microtubule sucking technique was used to study the adhesion of human hepatocarcinoma MHCC97-H cells to the full-length sense and antisense human KAI1 transfectants. Results The adhesion of each group of hepatocellular carcinoma cells measured by microtubule sucking system were MHCC97-HS group (678 · 4 ± 101 · 8), MHCC97-H- (1083 · 6 ± 113 · 8), MHCC97-H-pCI group (853 · 0 ± 105 · 4) and MHCC97-H parental cell group (834 · 6 ± 130 · 5) Cell number 25). The adhesion of MHCC97-HS cells to FN was significantly lower than that of MHCC97-H parental cells (P <0.01), and the adhesion of MHCC97-H-AS cells to FN was significantly increased (P <0 · 01), while the adhesion of MHCC97-H-pCI cells to FN had no significant change (P> 0.05). Conclusion The KAI1 gene may reduce the adhesion of liver cancer cells to extracellular matrix FN, thus inhibiting the initial steps of metastasis, and then inhibiting the invasion and metastasis of MHCC97-H hepatocarcinoma cells.