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目的:探讨中药“益肾汤”治疗IgA肾病(IgAN)的机制。方法:用“口服牛血清白蛋白联合尾静脉注射葡萄球菌肠毒素B”法复制小鼠IgAN模型。设正常照、模型照、益肾汤低浓度和高浓度组共4组。FQ-RT-PCR法检测小鼠肾组织MMP-9、TIMP-1mRNA表达、免疫组化SABC法检测小鼠肾组织MMP-9、TIMP-1的含量。结果:模型组小鼠肾间质MMP-9表达与正常组无统计学意义,而模型组TIMP-1表达则明显上调,有统计学意义(P<0·05)。益肾汤低浓度与高浓度组之间肾小管间质MMP-9、TIMP-1表达无统计学意义,但两组MMP-9表达均强于模型组(P<0·05),而TIMP-1的表达均弱于模型组(P<0·05)。结论:益肾汤可促进小鼠肾小管间质MMP-9的表达、抑制TIMP-1的表达、促进ECM的降解,进而延缓IgAN肾小球硬化的进展。
Objective: To explore the mechanism of “Yishen Decoction” in the treatment of IgA nephropathy (IgAN). METHODS: The mouse IgAN model was replicated using oral bovine serum albumin combined with tail vein injection of staphylococcal enterotoxin B. A total of 4 groups including normal exposure, model illumination, and Yishentang low concentration and high concentration group were established. FQ-RT-PCR method was used to detect the expression of MMP-9 and TIMP-1 mRNA in kidney tissue of mice, and immunohistochemical SABC method was used to detect the content of MMP-9 and TIMP-1 in kidney tissue of mice. Results: The expression of MMP-9 in the renal interstitium of the model group was not statistically significant compared with the normal group, but the expression of TIMP-1 was significantly up-regulated in the model group (P<0.05). There was no significant difference in the expression of MMP-9 and TIMP-1 in tubulointerstitium between the low and high concentrations of Yishentang, but the expression of MMP-9 in both groups was stronger than that in the model group (P<0.05). The expression of -1 was weaker than that of the model group (P < 0.05). Conclusion: Yishen Decoction can promote the expression of MMP-9, inhibit the expression of TIMP-1, promote the degradation of ECM, and delay the progression of IgAN glomerulosclerosis.