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[Objective] To establish the method for the quality control of Hibiscus mutabilis L. leaves. [Method] Microscopic identification and TLC were used to identify H. mutabilis leaves quantitatively. HPLC method was adopted to determine the content of active component rutin. [Result] Microscopic characteristics of H.mutabilis leaves were distinguishing. TLC spots were clear and well-separated. The linear range of rutin was 0.018 8-0.941 2 μg (r=0.999 6) with an average recovery rate of 97.97% (RSD=1.97%, n=6). The average content of rutin was 1.780 7 mg/g in 6 bathes of H.mutabilis leaves. [Conclusion] The established standard can be used to control the quality of H.mutabilis.
[Objective] To establish the method for the quality control of Hibiscus mutabilis L. leaves. [Method] Microscopic identification and TLC were used to identify H. mutabilis leaves quantitatively. HPLC method was adopted to determine the content of active component rutin. [Result TLC linear were clear and well-separated. The linear range of rutin was 0.018 8-0.941 2 μg (r = 0.999 6) with an average recovery rate of 97.97% (RSD = 1.97 %, n = 6). The average content of rutin was 1.780 7 mg / g in 6 bathes of H.mutabilis leaves. [Conclusion] The established standard can be used to control the quality of H.mutabilis.