笔者在1990《食用菌》第1期上介绍的用电烙铁打孔接种法后,读者纷纷来信询问具体做法,现再借贵刊一角作如下答复。在接种量大时使用电烙铁打孔接种,为了提高成品率,可在无菌室或其它设置的无菌区内操作;如采用开放接种,接种时间最好安排在深夜1～3点钟,此 The author in 1990, “edible mushrooms,” the first introduced on the use of electric iron perforated method of vaccination, the reader has a letter asked about the specific practices, is now the corner of your magazine for the following reply. Inoculation of large amount of use of electric iron punch vaccination, in order to improve the yield, can be sterile room or other settings within the aseptic zone operation; such as the use of open vaccination, vaccination time is best arranged in the late 1 to 3 o’clock, this