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本研究着重探讨内皮素对大鼠主动脉的收缩作用及对主动脉平滑肌细胞细胞周期及能量代谢的影响。浓度为1.0～18.0nmol/L的内皮素可引起大鼠主动脉产生浓度依赖性收缩,其EC_(50)为3.75±0.75nmol/L。正常对照组中平滑肌细胞S+G_2M期细胞的比例基本稳定在26.3％～29.6％的范围内;浓度为10.0和1000.0pmol/L的内皮素可使S+G_2M期细胞比例明显增高,上述变化呈良好的浓度依赖性(P<0.01);内皮素作用3～24h,随作用时间延长,S+G_2M期细胞比例逐步增多,呈明显的时间依赖性(P<0.01)。10.0pmol/L的内皮素作用10min后ATP含量增至对照组的139.05±1.11％(P<0.01);20min后ATP含量为对照组的65.25±5.09％(P<0.01),低于对照组水平。结果表明,内皮素具有缩血管作用及促平滑肌细胞增殖作用,上述作用可能在高血压的发病机制中有一定的意义。
This study focused on the effects of endothelin on rat aortic contractions and on the cell cycle and energy metabolism of aortic smooth muscle cells. The concentration of 1.0 ~ 18.0nmol / L of endothelin can cause rat aortic concentration-dependent contraction, the EC 50 (3.75 ± 0.75nmol / L). The proportion of cells in S phase and G 2 M phase in normal control group was basically stable in the range of 26.3% -29.6%. Endothelin at concentrations of 10.0 and 1000.0 pmol / L significantly increased the proportion of S + G 2 M phase cells in the control group (P <0.01). The effect of endothelin was 3 ~ 24h. With the extension of time, the percentage of cells in S phase increased gradually (P <0.01). ATP content increased to 139.05 ± 1.11% (P <0.01) of the control group after 10.0 minol / L endothelin treatment for 10 min, and to 65.25 ± 5.09% (P <0.01) of the control group after 20 min, which was lower than that of the control group . The results show that endothelin has a vasoconstrictive effect and promote smooth muscle cell proliferation, the above effect may have a certain significance in the pathogenesis of hypertension.