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取细粒棘球蚴囊液经离心、超滤浓缩,直接或放射碘标记后用于试验。将浓缩的囊液抗原免疫家兔后收集的抗血清通过与正常羊血清连接的溴化氰活化琼脂糖4B柱吸收,以除去抗羊血清蛋白抗体成分。试验血清包括从不同国家获得的细粒棘球绦虫、多房型棘球绦虫、猪肉绦虫、马来丝虫、班氏丝虫、盘尾丝虫、曼氏/埃及血吸虫、鞭虫、蛔虫、板口线虫患者血清及英国正常人血清。采用放射免疫沉淀试验、SDS-PAGE和免疫印渍试验进行囊液抗原性的分析和测定。
Echinococcus granulosus fluid taken centrifuged, ultrafiltration concentration, direct or radioactive iodine labeled for testing. The antiserum collected after immunization of the concentrated cystic antigen was absorbed by a column of cyanogen bromide-activated Sepharose 4B attached to normal goat serum to remove anti-sheep serum albumin antibody components. Test sera included Echinococcus granulosus, Echinococcus multilocularis, Taenia solium, Malayi filariasis, Bancroftian filariasis, Onchocerca volvulus, Schistosoma mansoni / Schistosoma mansoni, whipworm, roundworm, Oral nematode patient serum and British normal human serum. Radioimmunoprecipitation, SDS-PAGE and immunoblotting were used to analyze and determine the antigenicity of cyst fluid.