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目的建立一个小鼠转移性乳腺癌净化后骨髓移植模型,并探讨益康唑(Econazole,Ec)药物净化效果。方法将小鼠自发性乳腺癌细胞系 TSA 转染新霉素抗性基因(Neo~R 基因)后与小鼠骨髓细胞混合,体外短期培养净化;通过移植后造血恢复,肺转移性结节和小鼠的存活进行评估。结果接受放射治疗后经尾静脉注射 TSA 细胞的小鼠均可产生乳腺癌肺转移;Ec 体外净化后骨髓移植小鼠造血恢复没有明显延迟,移植后4周内未见肺转移性结节;小鼠的总生存状况明显改善。结论成功建立了 TSA 小鼠乳腺癌净化后骨髓移植模型,方法简单、易行、重复性好;Ec 可用于转移性乳腺癌净化后骨髓移植。
Objective To establish a mouse model of posttransplant bone marrow transplantation for metastatic breast cancer and explore the effect of Econazole (Ec) drug purification. Methods Mouse spontaneous breast cancer cell line TSA was transfected with neomycin resistance gene (Neo ~ R gene) and then mixed with mouse bone marrow cells and purified in vitro in short term. After hematopoietic recovery, lung metastatic nodules and Mice were evaluated for survival. Results The mice transplanted with TSA via tail vein after radiation therapy could produce lung metastasis of breast cancer. No significant delay was found in the recovery of hematopoietic cells after Ec purification in vitro, and lung metastatic nodules were not seen within 4 weeks after transplantation The overall survival of rats improved significantly. Conclusion The purified bone marrow transplantation model of TSA mouse breast cancer was successfully established. The method is simple, easy and reproducible. Ec can be used in bone marrow transplantation after the treatment of metastatic breast cancer.