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本文对40例军团病进行了前瞻性临床研究。作者做了一系列的化验检查,以便对军团病现代的实验诊断方法作出评价。对1979年3月～1981年12月期间收治的所有肺炎患者,常规进行军团病的特异性检测,包括血清抗体滴度(血清型1～6)、呼吸道标本直接免疫荧光试验(DFA)和选择性培养基培养。各种标本如胸水、痰液、气管内吸出液和肺组织刮取物等均在采集后两天内进行DFA 检测。对考虑为军团病感染的标本进行需氧菌和厌氧菌培养。气管内吸出液、胸水和肺组织作嗜肺性军团杆菌培养;痰液和
In this paper, 40 cases of Legionnaires’ disease were prospectively studied. The authors did a series of laboratory tests to evaluate modern laboratory diagnostics of Legionnaires’ disease. Specific detection of Legionnaires’ disease was routinely performed in all patients with pneumonia admitted between March 1979 and December 1981, including serum antibody titers (serotypes 1 to 6), direct immunofluorescence test (DFA) of respiratory specimens and selection Culture medium. A variety of specimens such as pleural effusion, sputum, endotracheal aspirates and lung tissue scraping material were collected within two days after the DFA test. Aerobically and anaerobically cultured specimens considered for Legionella disease infection. Tracheal aspiration, pleural effusion and lung tissue for pulmonary Legionella pneumophila culture; sputum and