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目的：研究丙型病毒性肝炎患者肝组织Ｆａｓ抗原的表达状况与肝细胞病理损伤的关系，探讨细胞凋亡在丙型肝炎发病机制中的作用和意义。方法：６９例丙型肝炎患者，均为血清抗－ＨＣＶＩｇＧ阳性或ＨＣＶＲＮＡ（ＰＣＲ）阳性，肝组织均为快速肝穿，福尔马林固定，连续切片，常规ＨＥ染色，按Ｋｎｏｄｅｌ方法计算ＨＡＩ积分前三项，根据Ｋｉｙｏｓｈｉ方法进行Ｆａｓ抗原半定量分析。结果：Ｆａｓ抗原在丙肝的阳性率为７４％，其中ＣＡＨ（８４．５％）。急肝（８３．４％）明显高于ＣＰＨ（６４．９％，Ｐ＜０．０５）。ＨＡＩ积分与Ｆａｓ抗原半定量分析显示，肝组织病理炎症积分越高，Ｆａｓ抗原表达量越高（Ｐ＜０．０５）。结论：Ｆａｓ抗原在丙型病毒性肝炎患者肝组织的表达与肝细胞的病理损伤明显相关，Ｆａｓ－ＦａｓＬ介导的细胞凋亡可能是丙型病毒性肝炎肝细胞坏死形式之一
Objective: To investigate the relationship between the expression of Fas antigen in liver tissue and hepatocyte pathological damage in patients with viral hepatitis C, and to explore the role and significance of apoptosis in the pathogenesis of hepatitis C virus. Methods: All the 69 patients with hepatitis C were positive for anti-HCVVIg or HCV RNA (positive for HCV RNA). All the liver tissues were rapidly hepatic perfusion and formalin fixation. Serial sections were stained with HE staining. The HAI score was calculated according to the Knodel method The first three, semi-quantitative analysis of Fas antigen according to Kiyoshi’s method. Results: The positive rate of Fas antigen in hepatitis C was 74%, of which CAH (84.5%). The acute liver injury (83.4%) was significantly higher than that of CPH (64.9%, P <0.05). Semi-quantitative analysis of HAI and Fas antigen showed that the higher the pathological inflammation score, the higher the expression of Fas antigen (P <0.05). Conclusion: The expression of Fas antigen in hepatic tissue of patients with hepatitis C virus is significantly correlated with the pathological damage of hepatocytes. Fas-FasL-mediated apoptosis may be one of the forms of hepatic necrosis of hepatitis C virus