目的:研究人参皂甙Rg3(GS-Rg3)联合Lewis Y(LeY)单克隆抗体对人子宫内膜移行上皮癌细胞株A431的增殖抑制作用及诱导其凋亡的作用。方法:体外培养A431细胞,分为对照组和实验组。采用MTT法观察GS-Rg3联合LeY单克隆抗体对A431细胞的增殖抑制作用;Hoechst33342荧光染色观察各组细胞凋亡的形态学情况;流式细胞术分析各组细胞周期变化及凋亡情况;western-blot方法检测各组Bcl-2和Bax蛋白的表达。结果:GS-Rg3和LeY单克隆抗体对A431细胞的增殖有明显的抑制作用,且以二者联合处理组作用最显著,呈协同作用,差异有统计学意义(P<0.05);经GS-Rg3和LeY单克隆抗体处理后,细胞周期停滞于G1期,S期无明显变化,A431细胞的凋亡率与对照组相比明显增加,以联合处理组最显著,差异有统计学意义(P<0.05);western-blot显示,GS-Rg3和LeY单克隆抗体作用A431细胞后,与对照组相比,Bcl-2表达减少,Bax表达增多,以联合作用组最为显著,差异有统计学意义(P<0.05)。结论:人参皂甙Rg3和Lewis Y单克隆抗体均能够抑制A431细胞的增殖,并通过下调Bcl-2和上调Bax诱导其凋亡,且二者具有协同增效作用。 AIM: To investigate the inhibitory effect of ginsenoside Rg3 (GS-Rg3) combined with Lewis Y (LeY) monoclonal antibody on the proliferation of human endometrial carcinoma cell line A431 and the induction of apoptosis. Methods: A431 cells were cultured in vitro and divided into control group and experimental group. The inhibitory effect of GS-Rg3 combined with LeY monoclonal antibody on the proliferation of A431 cells was observed by MTT assay. The morphological changes of apoptotic cells were observed by Hoechst33342 staining. The cell cycle and apoptosis were analyzed by flow cytometry -blot method was used to detect the expression of Bcl-2 and Bax in each group. Results: Monoclonal antibodies against GS-Rg3 and LeY significantly inhibited the proliferation of A431 cells, and the combination of the two showed the most significant synergistic effect with a significant difference (P <0.05). GS- Rg3 and LeY monoclonal antibody treatment, the cell cycle arrest in the G1 phase, S phase showed no significant change in the apoptotic rate of A431 cells compared with the control group was significantly increased to the joint treatment group was the most significant difference was statistically significant (P <0.05). Western-blot showed that after treated with GS-Rg3 and LeY monoclonal antibodies, the expression of Bcl-2 and Bax increased in A431 cells compared with the control group, the most significant effect was found in the combination group (P <0.05). CONCLUSION: Both ginsenoside Rg3 and Lewis Y monoclonal antibodies can inhibit the proliferation of A431 cells, and induce apoptosis by down-regulating Bcl-2 and up-regulating Bax, both of which have synergistic effects.