目的掌握苏州市自然界蚊虫中携带乙型脑炎病毒情况。方法 2013年7月份在苏州市采集蚊虫样本,利用乙型脑炎病毒PreM/C基因特异性引物进行real-time-PCR(RT-PCR),阳性样本通过C6/36细胞分离乙型脑炎病毒,应用Clustal X和MEGA 6生物软件完成阳性样本核苷酸同源性分析和进化树构建。结果共采集蚊虫1 055只,在三带喙库蚊中检出GⅠ型乙型脑炎病毒1株。结论首次证实苏州市蚊虫携带基因Ⅰ型乙型脑炎病毒,应在以后的监测和日常生活中加强防范。 Objective To understand the situation of entrapment of encephalitis virus in mosquitoes in nature in Suzhou City. Methods Mosquitoes samples were collected in Suzhou City in July 2013. Real-time-PCR (RT-PCR) was performed using PreM / C gene-specific primers of Japanese encephalitis virus (JEV). Positive samples were separated from Japanese encephalitis virus , Positive samples were analyzed for nucleotide homology and phylogenetic tree using Clustal X and MEGA 6 biological software. Results A total of 1 055 mosquitoes were collected and 1 strain of GⅠEJ was detected in Culex tritaeniorhynchus. Conclusion It was confirmed for the first time that mosquito-carrying genotype I Japanese encephalitis virus in Suzhou City should be strengthened in future monitoring and daily life.