骆驼刺与刺糖水提物对小鼠肠推进作用的研究

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目的研究骆驼刺与刺糖水提物对小鼠小肠运动的影响,为兽医临床防治动物胃肠道疾病筛选植物药及安全使用剂量选定提供依据。方法回流提取骆驼刺与刺糖水提剂,用硫酸阿托品与氯化氨甲酰甲胆碱分别建立抑制小肠运动和促进小肠运动的病理模型,骆驼刺及刺糖水提剂分别以高、中、低3种剂量给药,以葡聚糖蓝的推进距离与小肠总长的比值计算小肠推进率,观察骆驼刺及刺糖水提剂对小鼠小肠运动的影响。结果骆驼刺及刺糖水提物明显促进小鼠小肠运动的同时,拮抗阿托品对小肠运动的抑制,骆驼刺水提剂高、中、低剂量的促进率分别为22%、16.39%、16.07%,刺糖水提剂的促进率分别为30.83%、24.92%、25.12%。同时抑制氯化氨甲酰甲胆碱对小肠运动的促进作用,高、中、低剂量的骆驼刺水提剂抑制率分别为12.99%、23.2%、23.36%,刺糖水提剂的抑制率分别为8.61%、13.41%、27.47%。结论骆驼刺与刺糖水提剂对小鼠小肠运动具有双重调节作用。 Objective To study the effects of Alhagi sparsifolia and Prickly ash water extract on small intestine motility in mice and provide the basis for the veterinary clinical prevention and treatment of gastrointestinal diseases and selection of safe dosage. Methods The extraction of camellia pricklyash and prickly water extract with atropine sulfate and carbachol were used to establish the pathological model of inhibiting small intestine movement and intestinal motility respectively. Three dosages were given to calculate the small intestine propulsion rate by the ratio of the advancing distance of dextran blue and the total length of the small intestine. The effects of the camel thorn and the pricking water extract on the small intestine movement were observed. Results Alhagi sparsifolia and water extract significantly promote intestinal motility in mice, while antagonizing the inhibition of atropine on small intestine movement, camel acne water extract high, medium and low dose rates were 22%, 16.39%, 16.07%, respectively, Prickly sugar water extract promotion rate was 30.83%, 24.92%, 25.12%. At the same time, the inhibitory effect of carbachol on intestinal motility was inhibited. The inhibitory rates of high, medium and low doses of camel’s hydrogel were 12.99%, 23.2%, 23.36%, respectively For 8.61%, 13.41%, 27.47%. Conclusion The effects of camel thorn and baring sugar water extract on the regulation of intestinal motility in mice.
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