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土壤盐碱化是影响全球农业生产和生态环境的重要问题。在农田、轻度盐碱草地和重度盐碱草地设置样地以块茎种植菊芋,次年5月块茎萌发阶段取块茎样品测定丙二醛、游离脯氨酸、可溶性糖含量以及抗氧化酶活性并进行蛋白质组学分析,分析了萌芽菊芋块茎对盐碱土壤胁迫的生理响应。0—20 cm土层的电导率(表征土壤可溶盐含量)表明从农田到轻度、重度盐碱草地土壤盐碱胁迫逐渐增强,丙二醛含量变化反映出菊芋块茎受害程度逐渐增加,并且基于游离脯氨酸的渗透调节能力也在逐渐增强。蛋白质组学分析结果显示与遗传信息加工相关的差异蛋白数量最多(占28.75%)且多为表达上调,意味着DNA复制和转录、蛋白质合成和折叠的相关蛋白在响应盐碱胁迫中发挥关键作用。碳水化合物及多糖代谢(占15%)、氨基酸代谢(占11.25%)以及能量代谢(占7.5%)相关的差异蛋白数量也较多,说明调节物质代谢平衡在萌芽菊芋块茎应对盐碱土壤胁迫过程中有重要作用。这些结果为揭示萌芽菊芋块茎适应盐胁迫的生理机制奠定了基础。
Soil salinization is an important issue affecting the global agricultural production and ecological environment. In the fields of farmland, mild saline grassland and severe saline grassland, the tubers were planted for planting Jerusalem artichoke. In tuber germination stage in May next year, tuber samples were taken for determination of malondialdehyde, free proline, soluble sugar content and antioxidant enzyme activities The proteomics analysis was carried out to analyze the physiological responses of germinated Jerusalem artichoke tubers to saline-alkali soil stress. The conductivity of 0-20 cm soil layer (characterization of soil soluble salt content) showed that soil salinity-alkaline stress gradually increased from farmland to mild and severe saline-alkali grassland soil, and the change of malondialdehyde content reflected the gradual increase of Jerusalem artichoke tuber damage degree Permeability regulation based on free proline is also gradually increasing. Proteomics analysis showed that the largest number of differential proteins (28.75%) related to the processing of genetic information was mostly upregulated, which implied that DNA replication and transcription, protein synthesis and folding related proteins play a key role in response to salt-alkali stress . The number of differential proteins related to carbohydrate and polysaccharide metabolism (15%), amino acid metabolism (11.25%) and energy metabolism (7.5%) were also higher, indicating that the regulation of metabolic balance of substances in germinating Jerusalem artichoke tuber should cope with saline-alkali soil stress In an important role. These results laid the foundation for revealing the physiological mechanism of budding Jerusalem artichoke tubers to adapt to salt stress.