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目的 探讨在氯化锂-匹罗卡品诱导大鼠急性痫性发作模型中N-myc表达及其意义。方法 健康Wistar雄性大鼠45只,随机分为生理盐水对照组、地西泮干预组和致痫1 h～9 d组(根据致痫后的不同时点分7个组),共9组,每组5只。采用氯化锂-匹罗卡品联合腹腔注射急性致痫大鼠模型,常规HE染色观察实验大鼠脑组织的形态学改变,免疫组化染色法观察脑内N-myc表达产物分布及其含量变化。结果 致痫1d～9d组尤其致痫6d和9d两组可见到脑组织损伤性改变,海马区重于皮质区。生理盐水对照组N-myc几乎无表达,地西泮干预组低表达,致痫组表达增多,尤其以致痫1d组和致痫3d组最为明显。结论 急性痫性发作或癫痫持续状态可导致脑组织损伤性改变,以海马结构等易损区为明显。N-myc表达及其表达量可能与急性痫性发作后脑损伤及其受累部位有关。
Objective To investigate the expression of N-myc and its significance in lithium-pilocarpine-induced acute seizure model in rats. Methods Forty five Wistar male rats were randomly divided into normal saline control group, diazepam intervention group and 1 h to 9 d group (divided into 7 groups at different time points after epileptic seizures) 5 per group The model of acute epileptic rats was induced by intraperitoneal injection of lithium-pilocarpine. The morphological changes of brain tissue were observed by HE staining. The distribution and content of N-myc in brain were observed by immunohistochemical staining Variety. Results The damage of brain tissue was observed on the 1st and 9th day of epilepsy, especially on the 6th and 9th day after epilepsy. The hippocampus was heavier than the cortex. N-myc in the saline control group was almost no expression, low expression in the intervention group of diazepam, increased expression of epilepsy group, especially in the group of epilepsy 1d and the group of epilepsy 3d was the most obvious. Conclusions Acute epileptic seizures or status epilepticus can lead to brain damage, which is obvious in vulnerable areas such as hippocampus. The expression of N-myc and its expression may be related to brain injury and its affected sites after acute seizure.