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我们对西德Borstel研究所,荷兰皇家热带病研究所和美国佛罗里达州理工学院人工感染麻风菌的66只犰狳的127只眼球做了病理学研究。虽然所有的动物均由静脉按种了相似数量的麻风菌,但三处的动物的眼内病变程度却有相当明显的不同。为了今后对麻风眼病进一步防治,希望能制成较为一致的麻风眼病动物模型。我们对造成这些差异的可能因素做了分析。在Borstel和Florida二组动物中,眼内病变为轻度者分别为82％与87％,为重度者各为18％与13％。眼内病变的轻重与感染的时间长短以及动物肝脾内麻风菌的数量之间,未发现有意义的关系。而阿姆斯特丹组的动物,病变轻微者只占20％,而重度者却占80％。虽然眼内病变程度与感染时间短,以及动物肝内细菌量无明显关系,但与其脾内细菌的量有平行的关系。分析发现三个研究所在实验感染动物时,操作上有些差别,主要在于阿姆斯特丹研究所是在同一天用取自Borstel研究所一只犰狳脾内的麻风菌做了所有动物(15只)的接种。接种前未经过冷冻、复温,而且在接种时用超声波处理了菌的混悬液。此组动物眼的病变较重且一致性较高,可能与此有关。今后制做麻风动物模型时,可以参照这种处理。
We performed a pathological study of 127 eyes of 66 Armadillo larchs that were artificially infected with Leprosy at the Borstel Institute in West Germany, the Royal Institute of Tropical Diseases in the Netherlands and the Florida Institute of Technology in the United States. Although all animals were given a similar amount of leprosy by vein, the extent of intraocular lesions in the three animals was quite different. In order to further prevent and treat leprosy eyes in the future, it is hoped that a more consistent animal model of leprosy can be made. We analyze the possible causes of these differences. In the Borstel and Florida two groups of animals, the lesions were mild in 82% and 87%, respectively, and in severe cases 18% and 13%, respectively. No significant relationship was found between the severity of intraocular lesions and the duration of infection, as well as the amount of leprosy in the liver and spleen of animals. In the group of animals in Amsterdam, only mild lesions accounted for 20%, while severe ones accounted for 80%. Although the degree of intraocular lesions and infection time is short, as well as the amount of bacteria in animals no significant relationship between the liver, but the amount of bacteria in the spleen with parallel relationship. The analysis found differences in the operation of the three laboratories when animals were experimentally infected, mainly because the Amsterdam Institute did all animals (15) on the same day with leprosy taken from the spleen of an armadillo in the Borstel Institute Inoculation. Before inoculation, no freezing and rewarming were performed, and the suspension of the bacteria was ultrasonically treated at the time of inoculation. This group of animals heavier lesions and higher consistency may be related to this. In the future to make leprosy animal model, you can refer to this treatment.