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目的:探究柴胡皂苷D(SSD)对Aβ诱导大鼠肾上腺嗜铬细胞瘤细胞(PC12)tau蛋白磷酸化水平的影响。方法:将PC12细胞分为3组:对照组,用DMEM培养基常规培养;Aβ组,用含有10μmol/L Aβ25-35的DMEM培养基培养PC12细胞;SSD组,选择含有4μg/m L SSD+10μmol/L Aβ25-35的DMEM培养基共同培养PC12细胞,24 h后收集各组细胞;利用Western blot技术,检测对照组、Aβ组和SSD组PC12细胞中tau mRNA表达及蛋白表达水平的变化。结果:与对照组细胞相比,Aβ处理组tau蛋白磷酸化水平显著升高(P<0.05);而SSD组细胞tau蛋白磷酸化水平无明显改变(P>0.05);同时,与对照组相比较,Aβ处理组和SSD处理组细胞tau mRNA表达水平无明显变化(P>0.05),tau总蛋白水平无明显变化(P>0.05)。结论:在PC12细胞中,SSD可抑制Aβ25-35诱导PC12细胞tau蛋白的过度磷酸化。
Objective: To investigate the effect of saikosaponin D (SSD) on phosphorylation of tau in Aβ-induced adrenal pheochromocytoma cells (PC12). Methods: PC12 cells were divided into three groups: control group, which was cultured in DMEM medium; Aβ group, PC12 cells were cultured in DMEM medium containing 10μmol / L Aβ25-35; SSD group, containing 4μg / m L SSD + PC12 cells were co-cultured with 10|Ìmol / L A|Â25-35 DMEM medium and the cells were harvested 24 hours later. The expression of tau mRNA and protein in PC12 cells in control, A|Â and SSD groups were detected by Western blot. Results: Compared with the control group, phosphorylation of tau in Aβ group was significantly increased (P <0.05), while phosphorylation of tau protein in SSD group had no significant change (P> 0.05). Compared with the control group The levels of tau mRNA in Aβ treated group and SSD treated group showed no significant change (P> 0.05), while there was no significant change in total tau protein (P> 0.05). Conclusion: In PC12 cells, SSD inhibits Aβ25-35-induced hyperphosphorylation of tau in PC12 cells.