目的　寻找一种治疗内毒素血症及其并发症较为有效的途径。方法　从感染革兰阴性杆菌J7患者体内含有内毒素类脂A抗体的淋巴细胞中提取mRNA ,经反转录再从IgG重链Fd两端及轻链通用引物分别扩增Fab基因片段 ,依次插入到噬菌体载体 pCOMB3中 ,电穿孔转入大肠杆菌XL1 blue ,经辅助病毒VCSM13感染 ,重组噬菌体溶源裂解。结果　Fab抗体表达于噬菌体CPⅢ的N端 ,噬菌体抗体库的容量为 4 .8× 10 6 ,筛选出抗内毒素类脂A抗体 ,经LPS蛋白对噬菌体抗体库进行3轮淘选 ,使抗内毒素类脂A的特异性抗体得到 10 0倍的富集。结论　通过直接和竞争ELISA实验筛选出 3株结合活性好的特异性抗体 ,为下一步应用研究奠定了基础。 Objective To find a more effective way to treat endotoxemia and its complications. Methods mRNA was extracted from lymphocytes containing endotoxin A antibody in J7 patients infected with Gram-negative bacilli. The Fab gene fragments were amplified by reverse transcription from both ends of IgG heavy chain Fd and the universal primer of light chain, Into the phage vector pCOMB3, electroporated into E. coli XL1 blue, infected by helper virus VCSM13, recombinant phage lytic. Results The Fab antibody was expressed on the N terminus of the phage CPIII. The phage antibody library had a capacity of 4. 8 × 10 6. The anti-endotoxin A antibody was screened by lipopolysaccharide (LPS) Specific antibodies to the toxin lipid A give a 10Ox enrichment. Conclusion Three specific antibodies with good binding activity were screened by direct and competitive ELISA assays, which laid the foundation for further application research.