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通过十二年的耕作和施肥长期定位试验研究表明，苏南太湖稻麦轮作地区，长期休闲的农田０～５ｃｍ土层最高含氮量可达２．８９％，休闲田结合每季耕翻，全氮含量下降，只及最高含氮量的７０％，保持在２％左右。无论免耕与否，不施肥土壤（０～５ｃｍ）全氮含量为最高含氮量的５０％左右，单施化肥时为５２％～５６％，猪粪＋化肥为６３％，秸秆十化肥为６３％～７０％。不同耕作处理对土壤全氮、氮素矿化势、生物量氮和矿质氮在土层中的分布及含量有明显的影响。施肥，特别是配施有机肥对土壤全氮和土壤供氮容量的影响大于耕作对其的影响。固定态铵的含量不仅与土壤中的粘土矿物有关，施肥对其也有影响。
After 12 years of long-term experiment on tillage and fertilization, the maximum nitrogen content in 0-5cm soil layer of long-term fallow farmland in Taihu Lake in southern Jiangsu province was 2.89% Total nitrogen decreased, only the highest nitrogen content of 70%, remained at about 2%. No matter tillage or not, the content of total nitrogen in no-fertilization soil (0 ~ 5cm) was about 50% of the highest nitrogen content, that of fertilizer application was 52% ~ 56%, that of pig manure + fertilizer was 63% 63% ~ 70%. Different tillage treatments had significant effects on the distribution and content of soil total nitrogen, nitrogen mineralization potential, biomass nitrogen and mineral nitrogen in soil layers. Fertilization, especially with organic manure on soil total nitrogen and soil nitrogen supply capacity is greater than tillage on its impact. Fixed ammonium content is not only related to clay minerals in the soil, fertilization has an impact on it.