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目的检测血管紧张素Ⅱ2型受体基因的单核苷酸多态性(SNP)并探讨其与山东地区汉族男性高血压病(EH)之间的关系。方法应用直接测序的方法在随机选择的20例男性EH中对血管紧张素Ⅱ2型受体基因作SNP的筛查,同时在96例EH男性患者和107例正常对照男性人群中评价这些SNP与EH的关系。结果共发现了7个SNP,其中有4个SNP在本研究中初次发现。对其中2个SNP(A1675G和T1334C)进行病例对照研究,发现A1675G位点上男性EH患者中A等位基因频率与正常对照组相比差异有统计学意义(EH组A等位基因频率为49·0%,正常组为34·6%,P<0·05)。结论血管紧张素Ⅱ2型受体基因的A1675G单核苷酸多态性可能与山东地区男性EH患者相关。
Objective To detect the single nucleotide polymorphism (SNP) of angiotensin Ⅱ type 2 receptor gene and to explore its relationship with Han male hypertension (EH) in Shandong province. Methods Direct sequencing was used to screen SNP of angiotensin Ⅱ type 2 receptor gene in 20 randomly selected male EH patients. The SNPs were compared with EH in 96 male EH patients and 107 normal control male subjects. Relationship. Results A total of 7 SNPs were found, of which 4 SNPs were found for the first time in this study. A case-control study of two SNPs (A1675G and T1334C) showed that the frequency of allele A in male EH patients at A1675G site was significantly different from that in normal controls (frequency of allele A in EH group was 49 · 0%, normal group was 34.6%, P <0 · 05). Conclusion A1675G single nucleotide polymorphism of angiotensin Ⅱ type 2 receptor gene may be associated with male EH in Shandong Province.