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目的观察３００ｍＬ／Ｌ乙酸局部注射治疗裸鼠移植瘤的效果，并探讨其作用机制．方法应用人肝癌（ｈＨＣＣ）细胞悬液接种于Ｂａｌｂ／ｃｎｕ／ｎｕ裸鼠皮下建立移植瘤模型共１２只，分为３组，乙酸组５只注射３００ｍＬ／Ｌ乙酸０１５ｍＬ，乙醇组５只注射无水乙醇０１５ｍＬ，盐水组２只注射生理盐水０１５ｍＬ，２次／ｗｋ，共４次．观察移植瘤生长情况．结果乙酸组移植瘤全部结痂、脱落，而乙醇组除一只裸鼠移植瘤结痂、脱落外其余均明显缩小（Ｐ＜００５），盐水组移植瘤呈结节样进行性生长．病理学检查：乙酸组可见纤维组织未见肝癌细胞，乙醇组除大片组织坏死外可见散在的肝癌细胞团，盐水组肝癌细胞生长良好．结论３００ｍＬ／Ｌ乙酸较无水乙醇能产生更广泛的组织坏死，可以代替无水乙醇局部注射治疗ｈＨＣＣ．
Objective To observe the effect of local injection of 300 mL/L acetic acid in the treatment of transplanted tumors in nude mice and to explore its mechanism of action. Methods Human hepatocellular carcinoma (hHCC) cell suspension was inoculated into the subcutaneous Balb/cnu/nu nude mice to establish a total of 12 xenograft tumor models. The tumors were divided into 3 groups. The acetic acid group was injected with 300 mL/L acetic acid 0 15 mL, ethanol group 5 An injection of ethanol 0 15mL, saline group 2 only injected 0 15mL physiological saline, 2 times / wk, a total of 4 times. Observe the growth of the transplanted tumor. Results All transplanted tumors in the acetic acid group were crusted and shed, while those in the ethanol group were significantly smaller than those in the nude mice transplanted tumors (P < 0.05). The transplanted tumors in the saline group showed nodular growth. Pathological examination: In the acetic acid group, no hepatocarcinoma cells were seen in the fibrous tissue. In the ethanol group, scattered hepatoma cell masses were seen except for large tissue necrosis. The hepatoma cells in the saline group grew well. Conclusion 300 mL/L acetic acid can produce more extensive tissue necrosis than absolute ethanol, and can be used instead of ethanol injection to treat hHCC.