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目的观察前列腺癌患者外周血单个核细胞PSA mRNA及蛋白表达,探讨其与前列腺癌微转移的关系。方法取抗凝血,Ficoll离心分离单个核细胞,RT-PCR检测PSA mRNA表达,Western blot检测PSA蛋白表达。结果(1)血清PSA>40．0ng／ml者23例,两者均表达的19例,阳性符合率82．61％(19／23);血清PSA <20．0ng／ml者16例,两者均表达的5例,阳性符合率31．25％(5／16);(2)PSA mRNA及蛋白的阳性表达与临床分期、Gleason评分均呈正相关;(3)血清PSA<20．0ng／ml的16例患者随访18个月,1例PSA mRNA及蛋白均表达阳性者,于前列腺癌根治术后半年内死于癌转移。结论外周血单个核细胞PSA mRNA及蛋白的联合检测是判断前列腺癌微转移的有效手段之一,并可预示前列腺癌的病理分级和不良预后。
Objective To observe the expression of PSA mRNA and protein in peripheral blood mononuclear cells of patients with prostate cancer and to explore its relationship with micrometastasis of prostate cancer. Methods Anti-coagulation and mononuclear cells were isolated by Ficoll. PSA mRNA was detected by RT-PCR and PSA protein by Western blot. Results (1) Serum PSA> 40.0ng / ml in 23 cases, 19 cases were both positive, the positive coincidence rate was 82.61% (19/23); serum PSA <20.0ng / ml in 16 cases, (2) The positive expression of PSA mRNA and protein were positively correlated with clinical stage and Gleason score; (3) The serum PSA level was less than 20.0ng / ml of 16 patients were followed up for 18 months, one case of PSA mRNA and protein were positive, died of cancer metastasis within six months after radical prostatectomy. Conclusion The combined detection of PSA mRNA and protein in peripheral blood mononuclear cells is one of the effective methods to judge the micrometastasis of prostate cancer, and can predict the pathological grade and the poor prognosis of prostate cancer.