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角膜细胞显示方法,常用的有两种:一是采用小动物(通常用蛙)的新鲜角膜,用硝酸银处理后,制成压片标本,结果角膜细胞显阴性,即在棕黑色的背景下,细胞呈现空白区;另是采用大动物(通常用猫)的角膜,用炭酸银处理,经福尔马林液还原后,结果角膜细胞显阳性,即在淡黄色的背景下,角膜细胞呈现棕黑色。前一种方法,因为动物较小,一个角膜只能制成几张标本。后一种方法,动物虽较大,角膜体积也大,但由于角膜为凸的,纤维排列成板层,而细胞成多突状互相连接,位于纤维板层之间;如用切片的方法,不容易顺着
Corneal cells display methods, there are two commonly used: First, the use of small animals (usually with frog) of fresh cornea, treated with silver nitrate, made of compressed specimens, the results were negative corneal cells, that is, in the dark brown background , The cells showed a blank area; the other is the use of large animals (usually cats) of the cornea, treated with silver carbonate, after reduction by formalin, the results were positive corneal cells, that is, in the light yellow background, corneal cells Brown black. The former method, because the animal is small, can only make a cornea a few specimens. The latter method, although larger animals, the cornea volume is also large, but because the cornea is convex, the fibers arranged in the lamellae, and the cells into multiple protruding interconnected, located between the fibrin lamellae; such as the use of slicing method, not Easy to follow