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目的利用微卫星ＤＮＡ研究人乳腺癌裸鼠模型中的遗传稳定性及其早期发现微转移的方法。方法将人乳腺癌移植于裸鼠乳腺脂肪垫，提取组织ＤＮＡ，在３个微卫星位点（Ｄ１４Ｓ６８、Ｄ１８Ｓ６９、Ｄ２０Ｓ１９９）对人乳腺癌、裸鼠移植瘤及其转移灶进行ＰＣＲ分析。结果裸鼠移植瘤及其转移灶在３个位点与人乳腺癌具有相同的微卫星ＤＮＡ。结论裸鼠移植瘤及其转移灶来源于人乳腺癌。人乳腺癌在异种移植、裸鼠体内连续传代及转移，体外培养过程中具有遗传稳定性。本法是一种简单、敏感、特异性强的鉴别肿瘤转移细胞的方法。
Objective To use microsatellite DNA to study the genetic stability of human breast cancer in nude mice and its early detection of micrometastases. Methods Human breast cancer was transplanted into the mammary fat pad of nude mice, tissue DNA was extracted, and PCR analysis was performed on three microsatellite loci (D14S68, D18S69, D20S199) for human breast cancer, nude mice transplanted tumors and their metastases. Results The transplanted tumors and their metastases in nude mice had the same microsatellite DNA in human breast cancer at three sites. Conclusion Transplanted tumor and its metastasis in nude mice are derived from human breast cancer. Human breast cancer is continuously passaged and metastasized in xenotransplantation and nude mice and has genetic stability during in vitro culture. This method is a simple, sensitive, and specific method to identify tumor metastatic cells.