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目的:探讨中心体蛋白Nlp是否促进卵巢癌细胞增殖、黏附与侵袭。方法:利用转染技术构建Nlp高表达的卵巢癌SKOV3细胞系,采用MTT法、肿瘤细胞黏附、跨膜迁移分析和平板集落形成率检测,比较SKOV3、SKOV3/GFP和SKOV3/Nlp细胞系在增殖、黏附与侵袭能力方面的差异。结果:与对照细胞相比,SKOV3/Nlp细胞的生长加快,SKOV3/Nlp细胞克隆形成率显著上升(P<0.05)。SKOV3/Nlp细胞在30min及60min时黏附率均显著大于SKOV3/GFP及SKOV3细胞(P<0.01)。SKOV3/Nlp、SKOV3/GFP、SKOV3细胞迁移至膜下的数目分别为:640±69,316±25,314±37。与SKOV3/GFP、SK-OV3细胞相比,SKOV3/Nlp细胞的迁移能力显著增强(P<0.05)。结论:当Nlp表达增高后,会导致肿瘤细胞的增殖、黏附、侵袭等恶性表型增加,提示Nlp具有促进肿瘤发生发展的生物学作用。
Objective: To investigate whether central body protein Nlp promotes the proliferation, adhesion and invasion of ovarian cancer cells. Methods: The SKOV3 cell line with high Nlp expression was constructed by transfection technique. MTT assay, cell adhesion, transmembrane migration assay and colony formation assay were used to detect the proliferation of SKOV3, SKOV3 / GFP and SKOV3 / Nlp cell lines , Differences in adhesion and invasion ability. Results: Compared with the control cells, SKOV3 / Nlp cells grew faster and the colony formation rate of SKOV3 / Nlp cells increased significantly (P <0.05). The adhesion rate of SKOV3 / Nlp cells at 30min and 60min were significantly higher than that of SKOV3 / GFP and SKOV3 cells (P <0.01). The numbers of migration of SKOV3 / Nlp, SKOV3 / GFP and SKOV3 cells to sub-membrane were 640 ± 69,316 ± 25,314 ± 37, respectively. Compared with SKOV3 / GFP and SK-OV3 cells, the migration ability of SKOV3 / Nlp cells was significantly enhanced (P <0.05). CONCLUSION: When the expression of Nlp is increased, the malignant phenotypes such as proliferation, adhesion and invasion of tumor cells will increase, suggesting that Nlp has the biological function of promoting tumor development.