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[目的]建立同时快速测定功能食品中绿原酸、白藜芦醇、葛根素和芦荟甙的高效液相色谱法。[方法]样品经甲醇超声提取,提取液经甲醇-水(20︰80)稀释并高速离心后,上清液供HPLC分析。色谱柱为Kinetex C18柱(4.6 mm×100 mm,2.6μm),柱温25℃,采用甲醇-0.15%冰乙酸水溶液作流动相,梯度洗脱,流速0.60 ml/min,紫外检测。[结果]本法线性范围上限至少为2μg,相关系数大于0.999,检出限0.15～0.72 ng,平均回收率99.6%～113%。[结论]该法简便快速、准确灵敏,可用于功能食品中绿原酸、白藜芦醇、葛根素和芦荟甙的同时测定。
[Objective] The research aimed to establish a HPLC method for rapid determination of chlorogenic acid, resveratrol, puerarin and aloin in functional foods at the same time. [Method] The sample was extracted by methanol and the supernatant was subjected to HPLC analysis after the extract was diluted with methanol - water (20︰80) and centrifuged at high speed. The column was a Kinetex C18 column (4.6 mm × 100 mm, 2.6 μm). The column temperature was 25 ℃ and the mobile phase was methanol-0.15% glacial acetic acid. The mobile phase was gradient elution at a flow rate of 0.60 ml / min. [Results] The upper limit of the linear range of this method was at least 2μg, the correlation coefficient was greater than 0.999, the detection limit was 0.15 ~ 0.72 ng and the average recovery was 99.6% ~ 113%. [Conclusion] The method is simple, rapid, accurate and sensitive and can be used for the simultaneous determination of chlorogenic acid, resveratrol, puerarin and aloin in functional foods.