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目的建立同时测定消渴通络胶囊中芍药苷和丹酚酸B的测定方法。方法采用HPLC法,色谱柱:Phenomenex Luna C18(4.6 mm×250 mm,5μm),流动相:乙腈-0.1%磷酸梯度洗脱,流速:1 mL/min,检测波长:230 nm。结果芍药苷的线性范围为0.0629～0.629μg(r=0.9998),平均回收率为100.24%,RSD为1.01%;丹酚酸B的线性范围为0.2532～2.532μg(r=0.9999),平均回收率为100.41%,RSD为1.32%。结论 HPLC法简便、可靠、专属性强,可用于消渴通络胶囊的质量控制。
Objective To establish a method for simultaneous determination of paeoniflorin and salvianolic acid B in Xiaoke Tongluo Capsule. Methods The HPLC method was as follows: Phenomenex Luna C18 (4.6 mm × 250 mm, 5 μm), gradient elution with acetonitrile - 0.1% phosphoric acid, the flow rate was 1 mL / min and the detection wavelength was 230 nm. Results The linear range of paeoniflorin was 0.0629 ~ 0.629μg (r = 0.9998), the average recovery was 100.24%, RSD was 1.01%. The linear range of salvianolic acid B was 0.2532 ~ 2.532μg (r = 0.9999) Was 100.41% with a RSD of 1.32%. Conclusion The HPLC method is simple, reliable and specific. It can be used for quality control of Xiaoke Tongluo Capsule.