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以尾叶桉、窿缘桉的胚性愈伤组织为亲本材料，采用ＰＥＧ（聚乙二醇）融合法进行了原生质体融合研究，结果表明：影响桉树原生质体融合的主要因素为：融合液中ＰＥＧ 克分子量及浓度、Ｃａ２＋ 浓度。ＰＥＧ 以克分子量４ ０００、６ ０００ 较为合适，所用浓度分别为５０％ 与３５％ 时效果最好；融合液中０．４ ｍ ｏｌ／Ｌ浓度的Ｃａ２＋ 的加入有助于原生质聚集体及融合体百分率的提高。融合所用保温温度３０℃为宜。融合液中０．４ ｍ ｏｌ／Ｌ甘露醇的补充有利于原生质与融合体稳定性的保持。异核体核融合率以融合后培养３～５ ｄ 时百分率最高
The embryogenic callus of Eucalyptus urophylla and Eucalyptus urophylla was used as the parent material and protoplast fusion was carried out by PEG (polyethylene glycol) fusion method. The results showed that the main factors affecting the fusion of protoplast of Eucalyptus were: PEG molecular weight and concentration, Ca2 + concentration. PEG at the molecular weight of 4 000,6 000 is more appropriate, the best concentration of 50% and 35%, respectively, when the best effect; 0.4 ml ol / L concentration in the fusion solution of Ca2 + contribute to the protoplast aggregates and fusions Percentage increase. Fusion temperature of 30 ℃ is appropriate. Supplementation of 0.4 mol / L mannitol in the fusion solution favors the maintenance of the stability of protoplasm and fusion. The fusion rate of heterokaryotic nuclei reached the highest percentage when cultured 3-5 days after fusion