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目的:探讨神经纤毛蛋白-1(neuropilin-1,NRP-1)的小干扰RNA(small interfering RNA,siRNA)对人胃腺癌细胞株AGS某些生物学行为的影响。方法:通过脂质体lip-2000分别将NRP-1特异性siRNA、control siRNA转染入人胃腺癌AGS细胞株作为转染组、无意义序列组,加入PBS的细胞作为空白对照组,采用MTT法检测三组细胞24h、48h、72h的增殖情况;Transwell小室侵袭和迁徙实验检测细胞的迁徙和侵袭能力。结果:转染后,转染组细胞的增殖能力在24h、48h和72h与对照组相比,均显著下降(P=0.00)。小室迁徙及侵袭实验表明:与对照组相比,转染组的胃癌AGS细胞迁徙能力显著下降,差异具有统计学意义(P<0.05)。转染组的胃癌AGS细胞侵袭能力显著下降,差异具有统计学意义(P<0.05)。结论:Neuropilin-1的siRNA能够明显影响人胃癌细胞株AGS的某些生物学行为。
Objective: To investigate the effect of small interfering RNA (siRNA) targeting neuropilin-1 (NRP-1) on some biological behaviors of human gastric adenocarcinoma cell line AGS. METHODS: NRP-1 specific siRNA and control siRNA were transfected into human gastric adenocarcinoma AGS cell line by lipofectamine 2000 respectively. The transfected group and nonsignificant group were treated with PBS as control. MTT The proliferation of three groups of cells at 24h, 48h, 72h was detected by flow cytometry. The migration and invasion of cells were detected by Transwell chamber invasion and migration assay. Results: After transfection, the proliferation of transfected cells were significantly decreased compared with the control group at 24h, 48h and 72h (P = 0.00). The cell migration and invasion assay showed that compared with the control group, the migration ability of AGS cells in gastric cancer cells in transfection group was significantly decreased, with significant difference (P <0.05). The invasion ability of AGS cells in gastric cancer cells in transfection group was significantly decreased, the difference was statistically significant (P <0.05). Conclusion: Neuropilin-1 siRNA can significantly affect some biological behaviors of human gastric cancer cell line AGS.