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应用半巢式聚合酶链式反应（ＰＣＲ）从一例慢性ＨＢｓＡｇ携带者血清中扩增出ＨＢＶ前Ｓ１基因，将其克隆于噬菌体Ｍ１３ｍｐ１９中进行序列分析。结果发现：与同源性最好的ＨＢＶａｄｒ野生株相比，所测的１０个克隆均有替代和插入突变，９个克隆有缺失突变，１０个克隆的核苷酸变异率为５．０％～１７．０％，氨基酸的变异率为１３．０％～６０．０％；１０个克隆之间的核苷酸变异率为２．３％～２４．３％，氨基酸的变异率为１４．０％～６６．０％；１０个克隆中没有核苷酸序列一致的克隆。上述资料证实，慢性ＨＢｓＡｇ携带者体内的ＨＢＶ前Ｓ１基因具有高度的异质性。
The HBV preS1 gene was amplified from a serum of chronic HBsAg carriers by semi-nested polymerase chain reaction (PCR) and cloned into the phage M13mp19 for sequence analysis. The results showed that compared with the wild-type HBVadr wild-type strain, the 10 clones tested had substitution and insertional mutations, nine clones had deletion mutation, and the nucleotide variation rate of 10 clones was 5.0% ~ 17.0%, and the variation rate of amino acids was 13.0% ~ 60.0%. The nucleotide variation rate of 10 clones was 2.3% ~ 24.3%, and the mutation rate of amino acids was 14%. 0% ~ 66.0%. No clones with identical nucleotide sequences were found among the 10 clones. The above data confirm that the HBV pre-S1 gene in chronic HBsAg carriers has a high degree of heterogeneity.