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目的探讨PDCD4基因对小鼠T淋巴细胞亚群分化的影响。方法采用流式细胞术检测PDCD4基因缺失小鼠脾细胞和淋巴结细胞中的CD8+T和CD4+T,及其亚群Th1、Th2、Th17和调节性T细胞(Treg)的特异性标记分子,并分析各细胞亚群的比例变化。结果 pdcd4-/-小鼠脾脏和淋巴结中CD8+T细胞数量较野生型C57BL/6小鼠有所下降(P<0.05),CD4+T细胞及Th1细胞也呈下降趋势,差异无统计学意义(P>0.05);而PDCD4基因缺失后Th2和Th17的分化比例无显著变化(P>0.05);进一步分析CD4+T细胞群体中CD25+Foxp3+Treg的表达,发现pdcd4-/-小鼠CD25+Foxp3+Treg比例明显上调(P<0.05)。结论 PDCD4基因能够影响部分T淋巴细胞亚群的分化,PDCD4基因敲除小鼠中CD8+T细胞数量下降和CD25+Foxp3+Treg比例升高,提示PDCD4基因有可能在免疫调节方面起到一定作用。而PDCD4基因对于CD4+T以及Th1、Th2和Th17的分化并无明显作用。
Objective To investigate the effect of PDCD4 gene on the differentiation of T lymphocyte subsets in mice. Methods Flow cytometry was used to detect the specific markers of CD8 + T and CD4 + T in splenocytes and lymph node cells of PDCD4-deficient mice and their subsets of Th1, Th2, Th17 and regulatory T cells (Tregs) And analyze the changes of the proportion of each cell subpopulation. Results The number of CD8 + T cells in spleen and lymph node of pdcd4 - / - mice was decreased compared with that of wild type C57BL / 6 mice (P <0.05), CD4 + T cells and Th1 cells also showed a decreasing trend, with no significant difference (P> 0.05). However, there was no significant difference in the proportion of Th2 and Th17 after PDCD4 gene deletion (P> 0.05). Further analysis of CD25 + Foxp3 + Treg expression in CD4 + T cell population showed that CD25 + Foxp3 + Treg ratio was significantly increased (P <0.05). Conclusion The PDCD4 gene can affect the differentiation of some T lymphocyte subsets. The decrease of CD8 + T cells and the increase of CD25 + Foxp3 + Treg in PDCD4 knockout mice suggest that PDCD4 may play a role in immune regulation . The PDCD4 gene for CD4 + T and Th1, Th2 and Th17 differentiation did not have a significant effect.