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[目的]探讨β2肾上腺素受体(β2-adrenergic receptor,β2-AR)基因+46(G→A)位和+79(G→C)位单核苷酸多态性(single nucleotide polymorphisms,SNP)与河南汉族原发性高血压(essential hypertension,EH)的相关性。[方法]应用PCR-RFLP的方法,检测汉族高血压患者96例和正常血压者196例的β2-AR基因+46和+79位单核苷酸多态性,并运用卡方分析数据。[结果]两种SNPs基因型分布符合Hardy-Weinberg平衡,+46(G→A)的基因型分布在高血压和对照组的差异无统计学意义(P﹥0.05),+79(G→C)的基因型分布在高血压和对照组中全部是GG,不存在基因多态性。从而得知我国河南汉族人群中β2-AR基因46位点和79位点多态性与高血压病无相关性。但46位点在女性人群中差异有统计学意义(P﹤0.05),与高血压病相关。[结论]46位点可能是河南汉族女性群体原发性高血压遗传的易感性标志之一。
[Objective] To explore the relationship between single nucleotide polymorphisms (SNP) of +46 (G → A) and +79 (G → C) of β2-adrenergic receptor ) And essential hypertension (essential hypertension, EH) in Henan Han. [Methods] PCR-RFLP method was used to detect the polymorphisms at +46 and +79 of β2-AR gene in 96 Chinese Han patients with hypertension and 196 normal subjects. Chi-square analysis was used to analyze the data. [Results] The distribution of genotypes of two SNPs was in accordance with Hardy-Weinberg equilibrium. The genotype distribution of +46 (G → A) was not significantly different between hypertension and control group (P> 0.05) ) Genotype distribution in hypertension and control group are all GG, there is no genetic polymorphism. So we know that there is no correlation between the polymorphisms of the 46 sites and the 79 sites of β2-AR gene and hypertension in Henan Han population of our country. However, the 46-point difference in the female population was statistically significant (P <0.05), associated with hypertension. [Conclusion] The 46 locus may be one of the genetic markers of susceptibility to essential hypertension in Han Chinese women in Henan province.