在花生新鲜针叶和干叶ＤＮＡ提取过程中，应用ｐＨ值较低，无机盐浓度较高的提取缓冲液可沉淀蛋白质，其中加入２％的β－巯基乙醇可有效地防止次生代谢物质使ＤＮＡ变色。提取出的ＤＮＡ样品产率在９２．６～２１６．３１ｎｇ／ｍｇ．ｆｗ，ＤＮＡ质量和纯度较高，２６０ｎｍ／２８０ｎｍ光密度比值在１．８～１．９之间。所得ＤＮＡ可直接用于限制性内切酶酶切，并可用于随机引物ＰＣＲ扩增。该方法为花生分子生物学研究提供基础。 In the process of DNA extraction from fresh needles and dried leaves of peanut, the protein was precipitated by the extraction buffer with lower pH and higher inorganic salt concentration, and the addition of 2% β-mercaptoethanol could effectively prevent secondary metabolites DNA discoloration The yield of extracted DNA samples ranged from 92.6 to 216.31 ng / mg. fw, DNA quality and purity higher, 260nm / 280nm optical density ratio between 1.8 to 1.9. The resulting DNA can be used directly for restriction endonuclease digestion and can be used for random primer PCR amplification. This method provides the basis for the molecular biology of peanut.