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不同厂牌及批号的染料,其纯度亦不同,甚至差别很大。配制染液多用％浓度,即取包括杂质在内的染料重量与溶剂体积的百分比,而不按染料的实际含量计算。故常因所用染料含量不同而染色结果往往不一样。生物学染色工作者通常不使用定量分析方法检测染料的实际含量,只能从染色的实际效果来判定,很不方便.调试染液滴染滤纸法弥补了这一不足,在染色前即可做到心中有数,便于根据具体情况调整与判断染液,查找染色结果不良的原因。该法具有简便、迅速、经济等优点。从应用结果看,不仅可用于调试混合染液,提示各种色调的比例关系,而且还能用于检定某些单一染液的染色能力。值得提倡与探讨。
Different brands and lot number of the dye, its purity is different, and even very different. Preparation of dye concentration with more use, that is, including impurities, including dye weight and solvent volume percentage, rather than the actual content of dye calculated. Often due to the different dye content and dyeing results are often different. Biological dyeing workers usually do not use the quantitative analysis method to detect the actual content of the dye, which can only be judged by the actual result of the dyeing, which is very inconvenient.It can make up for the deficiency before the dyeing To the bottom of my heart, easy to adjust according to the specific circumstances and determine the dye, find the cause of poor staining results. The method is simple, rapid, economical and other advantages. From the application results, not only can be used to debug mixed dye solution, suggesting a variety of color ratio, but also can be used to test some of the dyeability of a single dye. Worth to promote and explore.