目的:建立HPLC法对净石灵胶囊中淫羊藿苷进行含量测定。方法:采用Diamonsil(250mm×4.6mm,5μm)柱;乙腈-水(30:70)为流动相;流速1.0ml·min~(-1);检测波长为270nm;结果:淫羊藿苷在0.40～2.00μg(r=0.9998)范围内,与峰面积值呈良好的线性关系。平均加样回收率为98.94%,RSD为0.5%(n=6)。结论:本法操作简便,准确,重复性好,适用于该制剂的质量控制。 Objective: To establish an HPLC method for the determination of icariin in Jingshiling Capsules. METHODS: A Diamonsil (250mm×4.6mm, 5μm) column was used; acetonitrile-water (30:70) was used as the mobile phase; the flow rate was 1.0ml·min-1 and the detection wavelength was 270nm; the result was icariin at 0.40. In the range of 2.002.00 μg (r=0.9998), there was a good linear relationship with the peak area value. The average recovery was 98.94% with an RSD of 0.5% (n=6). Conclusion: This method is simple, accurate and reproducible. It is suitable for the quality control of the preparation.