褪黑素是失眠及老年人常用的催眠药之一。近年来,一些犯罪分子利用其为作案工具,从而使之也出现在物证鉴定的检验项目中。本文报道一起涉及褪黑素的麻醉凶杀案的检验鉴定,以原体及代谢物作为中毒标记物,对褪黑素及其代谢物6-羟基褪黑素进行分析检验,并对案件信息作深度挖掘。使用日本岛津30A-LC液相色谱仪串联美国AB SCIEX公司API5500 QTRAP质谱仪,对送检样品进行测定化验,发现受害人血液褪黑素浓度超过正常起催眠作用的浓度达25倍以上。本文建立的液相色谱/串联质谱检验分析,方法简单、快速、有效,效果良好。 Melatonin is one of the commonly used hypnotics for insomnia and the elderly. In recent years, some criminals have used it as a tool for committing crimes, so that they have also appeared in the test items of physical evidence accreditation. This article reports the investigation and identification of a murine homicide involving melatonin. Using protoplasm and metabolites as markers of toxicity, the melatonin and its metabolite 6-hydroxy-melatonin were tested and the case information was further analyzed Dig Japan Shimadzu 30A-LC liquid chromatography series AB SCIEX Company API5500 QTRAP mass spectrometer, the test samples for testing and found that the victim's blood melatonin concentration than normal hypnotic effect of more than 25 times the concentration. The established liquid chromatography / tandem mass spectrometry test method, the method is simple, fast, effective, good effect.