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为了各地开展体外微量测定法调查恶性疟原虫的抗药性,研制了便于现场调查使用的培养基。将连续培养恶性疟原虫用的RPMI1640完全液体培养基用安瓿封装,冰瓶贮存.可供现场50d内使用。该培养基的效果与冰冻干燥培养基相似,明显优于WHO培养基。
In order to investigate the resistance of Plasmodium falciparum by in vitro micro assay, a medium for field investigation was developed. The RPMI 1640 complete liquid medium for continuous culture of P. falciparum was packaged in ampoules and stored in ice cylinders for use within 50 days of the site. The effect of this medium is similar to freeze-dried medium, which is significantly better than WHO medium.