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【目的】叶片是水稻理想株型的重要内容,叶片适度卷曲可以提高光合效率。对卷叶相关基因进行遗传分析和初步定位,为下一步的基因克隆与功能分析提供研究基础。【方法】利用EMS诱变雄性不育保持系宜香1B获得一份稳定遗传的叶片向内卷曲突变体,暂命名为rl(t)。在成熟期,测定野生型和rl(t)的主要农艺性状;在分蘖期,取野生型和rl(t)叶片用FAA固定液固定进行石蜡切片,同时,用野生型和rl(t)剑叶测定叶绿素含量;在抽穗期,利用Li-6400便携式光合仪测定10株抽穗期的野生型和rl(t)的光合参数;将rl(t)与野生型及日本晴杂交,观察F_1植株表型,对F_2表型分离进行χ~2测验,对突变体进行遗传分析。以rl(t)/日本晴的F_2群体为材料,利用BSA法进行定位。【结果】与野生型相比,突变体叶片向内卷曲明显,叶片更加直立,叶色变深,其他主要农艺性状均有不同程度降低。光合特性分析表明,突变体比野生型具有更高的光合色素含量,但光合效率没有明显差异。叶片组织切片观察表明,突变体中泡状细胞变小可能是导致叶片卷曲的主要原因。遗传分析表明,该突变体受一对隐性核基因控制,利用突变体与日本晴的F_2群体进行基因定位,最终将该基因定位在第7染色体长臂InDel标记Ind3和Ind4间610 kb的物理区间。【结论】rl(t)叶片内卷是由于近轴面泡状细胞面积减小。RL(t)定位区间内未见卷叶相关基因报道,推测RL(t)可能是一对新基因。
【Objective】 Leaf is an important part of rice plant type. Moderate curling leaves can improve photosynthetic efficiency. Genetic analysis and preliminary mapping of leaf-related genes provide the basis for the next step of gene cloning and functional analysis. 【Method】 A stable inherited leaf curl mutant was tentatively named rl (t) by EMS mutagenesis male sterile maintainer Yixiang 1B. At the mature stage, the main agronomic traits of wild type and rl (t) were determined. At the tillering stage, the wild type and rl (t) leaves were fixed with FAA fixative for paraffin sectioning. At the same time, The leaf chlorophyll content was measured at heading stage. The photosynthetic parameters of wild type and rl (t) at 10 heading date were measured by Li-6400 portable photosynthesis apparatus. The rl (t) was crossed with wild type and Nipponbare to observe the phenotype of F 1 plant , The F_2 phenotype of the separation of χ ~ 2 test for genetic analysis of the mutant. The F2 population of rl (t) / Nipponbare was used as the material, and the BSA method was used for the mapping. 【Result】 Compared with the wild type, the leaves of the mutants were curled inwardly, the leaves were more upright, the leaf color became darker, and the other agronomic traits decreased to some extent. Photosynthetic characteristics analysis showed that the mutant had higher photosynthetic pigment content than the wild type, but no significant difference in photosynthetic efficiency. Observation of leaf tissue sections showed that the decrease of foam cells in the mutant may be the main reason leading to leaf curling. Genetic analysis showed that the mutant was controlled by a pair of recessive nuclear genes. The mutant was mapped with the F_2 population of Nipponbare, and the gene was mapped to the 610 kb physical region of InDel marker Ind3 and Ind4 on chromosome 7 . 【Conclusion】 Leaf volume of rl (t) is due to the reduction of the area of bubble cells in the paraxial plane. RL (t) positioning interval no coil leaf-related genes reported speculated that RL (t) may be a pair of new genes.