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Objective To investigate the effect of insuline-like growth factor-Ⅰ(IGF-Ⅰ) on progesterone genesis and regulation.Methods Cytotrophoblast cells were collected by trypsin-collagenase digestion and percoll gradient centrifugation for primary culture.After stimulated with different concentrations(1 00 μg/ml,10 μg/ml,1μg/ml,0.1 μg/ml) of IGF-Ⅰat the same time and with different duration(12 h, 24 h, 48 h,72 h) of IGF-Ⅰ with the same concentration, progesterone levels in the media were measured by radioimmunoassay.Simultaneously,semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) was applied to determine the expression of low density lipoprotein receptor (LDLR) mRNA.Results Progesterone levels correlated positively with IGF-Ⅰalong with the IGF-Ⅰconcentration increasing,progesterone level began to increase at 12 h,and reached the climax at 48 h when cultured with 100 μg/L IGF-Ⅰ. The expression of LDLR mRNA was detectable in every group and accordant with variation of progesterone level.Conclusion Progesterone secretion has time- and dose-dependent effect on IGF-Ⅰ,and IGF-Ⅰcan up-regulate the expression of LDLR mRNA. IGF-Ⅰmay play an important role in promoting secretion of progesterone in trophoblast cells.