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目的探讨柴芍和胃颗粒对大鼠免疫性胃炎的保护作用及其机制。方法以弗氏完全佐剂制备免疫性胃炎大鼠模型。将模型大鼠随机分为模型组,三九胃泰颗粒阳性对照组,柴芍和胃颗粒(0.8、1.6、3.2 g/kg剂量)组,另设对照组;于开始造模后第28天ig给药,连续给药28 d。在造模不同时间称大鼠体质量,给药结束后检测大鼠血清中NO、白细胞介素-2(IL-2)水平,胃组织匀浆谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MDA)水平,观察胃组织病理学改变。结果与模型组相比,柴芍和胃颗粒低、中、高剂量组大鼠血清NO水平分别降低20.53%、63.95%、69.85%;使大鼠胃黏膜组织匀浆中GSH-Px的活性分别增加16.11%、16.11%、30.33%,MDA水平分别降低5.37%、25.4%、34.69%;使大鼠血清IL-2水平有增加的趋势,以高剂量组作用明显,但与模型组相比差异不显著。病理组织学观察可见,模型组大鼠胃黏膜的固有层内出现结缔组织增生及淋巴小结增多,黏膜肌层增厚,平滑肌纤维走向紊乱,炎性细胞浸润严重;柴芍和胃颗粒低、中、高剂量组大鼠黏膜层增生及其炎性浸润均有不同程度的减轻和恢复。结论柴芍和胃颗粒对大鼠胃黏膜损伤具有一定的保护作用,其机制可能与其增强抗氧化酶活性、减轻自由基损伤和抑制脂质过氧化物反应有关。
Objective To investigate the protective effect and its mechanism of Chai Shao Wei Wei (Stomach Granule) on immune gastritis in rats. Methods Freund’s complete adjuvant was used to prepare rat model of immune gastritis. The rats were randomly divided into three groups: model group, Sanjiueweitai granule positive control group, Chaishao and stomach granule (0.8,1.6,3.2 g / kg dose) group, and another control group. On the 28th day after model establishment ig administration, continuous administration 28 d. The rat body weight was measured at different time points after modeling. The level of NO, interleukin-2 (IL-2) in serum, glutathione peroxidase (GSH-Px ) Activity, malondialdehyde (MDA) levels were observed gastric pathological changes. Results Compared with the model group, the levels of NO in serum of CHAO and GGH groups were decreased by 20.53%, 63.95% and 69.85%, respectively. The activities of GSH-Px in gastric homogenate The levels of IL-2 in serum increased by 16.11%, 16.11% and 30.33%, respectively, while the level of MDA decreased by 5.37%, 25.4% and 34.69%, respectively. Not obvious. Histopathological observation showed that there was an increase of connective tissue hyperplasia and lymphoid nodules in the lamina propria of gastric mucosa in model group, thickening of muscular layer of mucosa, disturbance of smooth muscle fibers and serious inflammatory cell infiltration. , High-dose group of mucosal hyperplasia and inflammatory infiltration have different degrees of reduction and recovery. Conclusions Chai Shao He Wei granules can protect gastric mucosal injury in rats. The mechanism may be related to the enhancement of antioxidant enzyme activity, the reduction of free radical damage and the inhibition of lipid peroxidation.