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目的:研究阿霉素前体药(PADM)对人胃癌细胞系MGC-803生长以及ERK1/2信号通路的影响。方法:培养MGC-803人胃癌细胞系,检测组织蛋白酶B的表达、PADM及阿霉素(ADM)对细胞生长的抑制作用、p-ERK1/2、ERK1/2和β-actin的表达,以及细胞周期。结果:MGC-803细胞系中组织蛋白酶B表达丰富;PADM和ADM对细胞生长呈剂量依赖性抑制作用;处理48 h时,PADM的半数抑制浓度(IC50)为14.9μmol/L,是ADM(IC50=4.9μmol/L)的3.04倍。与ADM相比,PADM显著下调p-ERK1/2水平,并使细胞周期停滞在G2/S期。结论:PADM可通过ERK1/2途径诱导细胞死亡。PADM与ADM抗肿瘤机制可能不同。
Objective: To study the effects of adriamycin prodrug (PADM) on the growth of human gastric cancer cell line MGC-803 and the ERK1 / 2 signaling pathway. Methods: MGC-803 human gastric cancer cell line was cultured and the expression of cathepsin B, the inhibitory effect of PADM and adriamycin on the cell growth, the expression of p-ERK1 / 2, ERK1 / 2 and β-actin cell cycle. Results: The expression of cathepsin B in MGC-803 cell line was abundant. PADM and ADM inhibited cell growth in a dose-dependent manner. The IC50 of PADM was 14.9 μmol / L at 48 h, = 4.9 μmol / L). Compared with ADM, PADM significantly down-regulated the level of p-ERK1 / 2 and arrested the cell cycle in G2 / S phase. Conclusion: PADM can induce cell death via ERK1 / 2 pathway. The anti-tumor mechanisms of PADM and ADM may be different.