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目的:探讨类风湿关节炎(rheumatoid arthritis,RA)中葡萄糖-6-磷酸异构酶(glucose-6-phosphate isomerase,GPI)的检测及其临床意义。方法:用酶联免疫吸附试(ELISA)检测血清中GPI,其中40例RA患者血清中GPI浓度为(2.67±2.48)μg·ml-1,20例其他免疫疾病患者血清中GPI浓度(0.094±0.063)μg·ml-1、15例健康人对照组GPI为(0.091±0.062)μg·ml-1,RA患者同时还进行了类风湿因子(RF)、血沉(ESR)等检测。结果:RA活动组与RA非活动组比较有统计学意义(P<0.05)。通过回归分析发现关节肿胀、疼痛与GIP浓度正相关。GPI抗原对RA检测的敏感性为63.5%,特异性为96.3%。结论:GPI在部分RA病人血清中显著升高,有可能成为诊断RA及判断其疾病活动性的一个新指标。
Objective: To investigate the detection of glucose-6-phosphate isomerase (GPI) in rheumatoid arthritis (RA) and its clinical significance. Methods: Serum GPI was detected by enzyme-linked immunosorbent assay (ELISA). The serum GPI level in 40 patients with RA was (2.67 ± 2.48) μg · ml-1, and the GPI in 20 patients with other immune diseases were 0.094 ± 0.063) μg · ml-1. The GPI of 15 healthy people was (0.091 ± 0.062) μg · ml-1. RA patients were also tested for rheumatoid factor (RF) and ESR. Results: The RA activity group and RA inactive group were statistically significant (P <0.05). By regression analysis, it was found that there was a positive correlation between swelling and pain in the joints. The GPI antigen has a sensitivity of 63.5% for RA detection and a specificity of 96.3%. Conclusion: The GPI is significantly increased in sera of some RA patients, which may become a new index for diagnosing RA and judging its disease activity.