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五分类血细胞分析仪作为全血细胞分析的初筛手段应用于临床,在出现异常细胞提示或报警时,须进行手工镜检加以确定,但手工镜检法易受主观因素影响、精密度低。流式细胞术通过特异单克隆抗体对细胞进行识别,结果准确、重复性好,其结合多抗体组合进行外周血白细胞分类计数已在多种疾病中应用。本文就流式细胞术分类计数白细胞在临床中的应用予以综述。
As a screening method of whole blood cell analysis, the five-category hematology analyzer is applied to clinic. When abnormal cell tips or alarms are found, manual inspection should be carried out to determine the abnormal cells. However, the manual examination is susceptible to subjective factors and has low precision. Flow cytometry by specific monoclonal antibodies to identify cells with accurate results, good reproducibility, combined with multi-antibody combination of peripheral blood leukocyte count has been used in a variety of diseases. This article reviews the application of flow cytometry in the clinical classification of white blood cells.